The zebrafish sperm proteome

Jayme Cohen-Krais; Carlo Martins; Jamie Bartram; Zoe Crighton; Jean-Charles de Coriolis; Alice Godden; Daniel Marcu; Weronika Robak; Gerhard Saalbach; Simone Immler

doi:10.1002/pmic.202400310

The zebrafish sperm proteome

Jayme Cohen-Krais, Carlo Martins, Jamie Bartram, Zoe Crighton, Jean-Charles de Coriolis, Alice Godden, Daniel Marcu, Weronika Robak, Gerhard Saalbach, Simone Immler

Research output: Contribution to journal › Article › peer-review

Abstract

One of the key processes that forms the basis of fertilisation is the tight interaction between sperm and egg. Both sperm and egg proteomes are known to evolve and diverge rapidly even between closely related species. Understanding the sperm proteome therefore provides key insights into the proteins that underpin the mechanisms involved during fertilisation and the fusion between sperm and egg, and how they can differ across individuals of the same species. Despite being a commonly used model organism for reproductive research, little is currently understood about the zebrafish Danio rerio sperm proteome. We performed nanoLC-MS/MS proteomics analysis after off-line sample fractionation with six pooled samples containing sperm from ten males each. We confidently identified 5410 proteins, from which a total of 3900 GeneIDs were generated leading to 1720 Gene Ontology terms.

Original language	English
Article number	e202400310
Journal	Proteomics
Early online date	31 Dec 2024
DOIs	https://doi.org/10.1002/pmic.202400310
Publication status	E-pub ahead of print - 31 Dec 2024

Keywords

Danio rerio
fertilisation
fertility
proteomics
sperm function
sperm-egg interaction

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10.1002/pmic.202400310Licence: CC BY

https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/pmic.202400310Licence: CC BY

Cite this

@article{44d261df50534f8ebb5a54008e4cc978,

title = "The zebrafish sperm proteome",

abstract = "One of the key processes that forms the basis of fertilisation is the tight interaction between sperm and egg. Both sperm and egg proteomes are known to evolve and diverge rapidly even between closely related species. Understanding the sperm proteome therefore provides key insights into the proteins that underpin the mechanisms involved during fertilisation and the fusion between sperm and egg, and how they can differ across individuals of the same species. Despite being a commonly used model organism for reproductive research, little is currently understood about the zebrafish Danio rerio sperm proteome. We performed nanoLC-MS/MS proteomics analysis after off-line sample fractionation with six pooled samples containing sperm from ten males each. We confidently identified 5410 proteins, from which a total of 3900 GeneIDs were generated leading to 1720 Gene Ontology terms.",

keywords = "Danio rerio, fertilisation, fertility, proteomics, sperm function, sperm-egg interaction",

author = "Jayme Cohen-Krais and Carlo Martins and Jamie Bartram and Zoe Crighton and {de Coriolis}, Jean-Charles and Alice Godden and Daniel Marcu and Weronika Robak and Gerhard Saalbach and Simone Immler",

note = "Data Availability Statement: The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD056057 and https://doi.org/10.6019/PXD056057. Funding information: This study was funded by grants from the Natural Environment Research Council (NE/S011188/1) and the European Research Council (SELECTHAPLOID-101001341) to SI.",

year = "2024",

month = dec,

day = "31",

doi = "10.1002/pmic.202400310",

language = "English",

journal = "Proteomics",

issn = "1615-9853",

publisher = "Wiley",

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T1 - The zebrafish sperm proteome

AU - Cohen-Krais, Jayme

AU - Martins, Carlo

AU - Bartram, Jamie

AU - Crighton, Zoe

AU - de Coriolis, Jean-Charles

AU - Godden, Alice

AU - Marcu, Daniel

AU - Robak, Weronika

AU - Saalbach, Gerhard

AU - Immler, Simone

N1 - Data Availability Statement: The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD056057 and https://doi.org/10.6019/PXD056057. Funding information: This study was funded by grants from the Natural Environment Research Council (NE/S011188/1) and the European Research Council (SELECTHAPLOID-101001341) to SI.

PY - 2024/12/31

Y1 - 2024/12/31

N2 - One of the key processes that forms the basis of fertilisation is the tight interaction between sperm and egg. Both sperm and egg proteomes are known to evolve and diverge rapidly even between closely related species. Understanding the sperm proteome therefore provides key insights into the proteins that underpin the mechanisms involved during fertilisation and the fusion between sperm and egg, and how they can differ across individuals of the same species. Despite being a commonly used model organism for reproductive research, little is currently understood about the zebrafish Danio rerio sperm proteome. We performed nanoLC-MS/MS proteomics analysis after off-line sample fractionation with six pooled samples containing sperm from ten males each. We confidently identified 5410 proteins, from which a total of 3900 GeneIDs were generated leading to 1720 Gene Ontology terms.

AB - One of the key processes that forms the basis of fertilisation is the tight interaction between sperm and egg. Both sperm and egg proteomes are known to evolve and diverge rapidly even between closely related species. Understanding the sperm proteome therefore provides key insights into the proteins that underpin the mechanisms involved during fertilisation and the fusion between sperm and egg, and how they can differ across individuals of the same species. Despite being a commonly used model organism for reproductive research, little is currently understood about the zebrafish Danio rerio sperm proteome. We performed nanoLC-MS/MS proteomics analysis after off-line sample fractionation with six pooled samples containing sperm from ten males each. We confidently identified 5410 proteins, from which a total of 3900 GeneIDs were generated leading to 1720 Gene Ontology terms.

KW - Danio rerio

KW - fertilisation

KW - fertility

KW - proteomics

KW - sperm function

KW - sperm-egg interaction

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U2 - 10.1002/pmic.202400310

DO - 10.1002/pmic.202400310

M3 - Article

JO - Proteomics

JF - Proteomics

SN - 1615-9853

M1 - e202400310

ER -

The zebrafish sperm proteome

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Keywords

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Understanding the role of selection at the gametic level in adaptation to changing environments

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The zebrafish sperm proteome

Abstract

Keywords

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Understanding the role of selection at the gametic level in adaptation to changing environments

Cite this