Three-dimensional rendering of otolith growth using phase contrast synchrotron tomography

James Mapp, Mark Fisher, Robert Atwood, Duncan Bell, Mark Greco, Sally Songer, Ewan Hunter

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We present a three-dimensional computer reconstruction of a plaice (Pleuronectes platessa) otolith from data acquired by the Diamond Light synchrotron, beamline I12, X-ray source, a high energy (53 – 150 keV) source particularly well suited to the study of dense objects. Our data allowed non-destructive rendering of otolith structure, and for the first time allows examination of otolith annuli (internal ring structures) to be analysed in X-ray tomographic images.
Original languageEnglish
Pages (from-to)2075–2080
Number of pages6
JournalJournal of Fish Biology
Issue number5
Early online date13 Apr 2016
Publication statusPublished - May 2016


  • 3-D Reconstruction
  • Fish Ageing
  • Otolith Growth
  • Phase Contrast
  • Synchrotron Tomography

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