Time- and concentration-dependent changes in gene expression induced by benzo(a)pyrene in two human cell lines, MCF-7 and HepG2

Sarah L Hockley, Volker M Arlt, Daniel Brewer, Ian Giddings, David H Phillips

Research output: Contribution to journalArticlepeer-review

96 Citations (Scopus)

Abstract

The multi-step process of carcinogenesis can be more fully understood by characterizing gene expression changes induced in cells by carcinogens. In this study, expression microarrays were used to monitor the activity of 18,224 cDNA clones in MCF-7 and HepG2 cells exposed to the carcinogen benzo(a)pyrene (BaP) or its non-carcinogenic isomer benzo(e)pyrene (BeP). Time and concentration gene expression effects of BaP exposure have been assessed and linked to other measures of cellular stress to aid in the identification of novel genes/pathways involved in the cellular response to genotoxic carcinogens.
Original languageEnglish
Article number260
JournalBMC Genomics
Volume7
DOIs
Publication statusPublished - 16 Oct 2006

Keywords

  • Benzo(a)pyrene
  • Cell Cycle
  • Cell Line
  • Cell Survival
  • DNA Adducts
  • Gene Expression
  • Gene Expression Profiling
  • Humans
  • Principal Component Analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Time Factors
  • Tumor Suppressor Protein p53

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