Time- and concentration-dependent changes in gene expression induced by benzo(a)pyrene in two human cell lines, MCF-7 and HepG2

Sarah L Hockley; Volker M Arlt; Daniel Brewer; Ian Giddings; David H Phillips

doi:10.1186/1471-2164-7-260

Time- and concentration-dependent changes in gene expression induced by benzo(a)pyrene in two human cell lines, MCF-7 and HepG2

Sarah L Hockley, Volker M Arlt, Daniel Brewer, Ian Giddings, David H Phillips

Research output: Contribution to journal › Article › peer-review

106 Citations (Scopus)

Abstract

The multi-step process of carcinogenesis can be more fully understood by characterizing gene expression changes induced in cells by carcinogens. In this study, expression microarrays were used to monitor the activity of 18,224 cDNA clones in MCF-7 and HepG2 cells exposed to the carcinogen benzo(a)pyrene (BaP) or its non-carcinogenic isomer benzo(e)pyrene (BeP). Time and concentration gene expression effects of BaP exposure have been assessed and linked to other measures of cellular stress to aid in the identification of novel genes/pathways involved in the cellular response to genotoxic carcinogens.

Original language	English
Article number	260
Journal	BMC Genomics
Volume	7
DOIs	https://doi.org/10.1186/1471-2164-7-260
Publication status	Published - 16 Oct 2006

Keywords

Benzo(a)pyrene
Cell Cycle
Cell Line
Cell Survival
DNA Adducts
Gene Expression
Gene Expression Profiling
Humans
Principal Component Analysis
Reverse Transcriptase Polymerase Chain Reaction
Time Factors
Tumor Suppressor Protein p53

Access to Document

10.1186/1471-2164-7-260Licence: CC BY

Cite this

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title = "Time- and concentration-dependent changes in gene expression induced by benzo(a)pyrene in two human cell lines, MCF-7 and HepG2",

abstract = "The multi-step process of carcinogenesis can be more fully understood by characterizing gene expression changes induced in cells by carcinogens. In this study, expression microarrays were used to monitor the activity of 18,224 cDNA clones in MCF-7 and HepG2 cells exposed to the carcinogen benzo(a)pyrene (BaP) or its non-carcinogenic isomer benzo(e)pyrene (BeP). Time and concentration gene expression effects of BaP exposure have been assessed and linked to other measures of cellular stress to aid in the identification of novel genes/pathways involved in the cellular response to genotoxic carcinogens.",

keywords = "Benzo(a)pyrene, Cell Cycle, Cell Line, Cell Survival, DNA Adducts, Gene Expression, Gene Expression Profiling, Humans, Principal Component Analysis, Reverse Transcriptase Polymerase Chain Reaction, Time Factors, Tumor Suppressor Protein p53",

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T1 - Time- and concentration-dependent changes in gene expression induced by benzo(a)pyrene in two human cell lines, MCF-7 and HepG2

AU - Hockley, Sarah L

AU - Arlt, Volker M

AU - Brewer, Daniel

AU - Giddings, Ian

AU - Phillips, David H

PY - 2006/10/16

Y1 - 2006/10/16

N2 - The multi-step process of carcinogenesis can be more fully understood by characterizing gene expression changes induced in cells by carcinogens. In this study, expression microarrays were used to monitor the activity of 18,224 cDNA clones in MCF-7 and HepG2 cells exposed to the carcinogen benzo(a)pyrene (BaP) or its non-carcinogenic isomer benzo(e)pyrene (BeP). Time and concentration gene expression effects of BaP exposure have been assessed and linked to other measures of cellular stress to aid in the identification of novel genes/pathways involved in the cellular response to genotoxic carcinogens.

AB - The multi-step process of carcinogenesis can be more fully understood by characterizing gene expression changes induced in cells by carcinogens. In this study, expression microarrays were used to monitor the activity of 18,224 cDNA clones in MCF-7 and HepG2 cells exposed to the carcinogen benzo(a)pyrene (BaP) or its non-carcinogenic isomer benzo(e)pyrene (BeP). Time and concentration gene expression effects of BaP exposure have been assessed and linked to other measures of cellular stress to aid in the identification of novel genes/pathways involved in the cellular response to genotoxic carcinogens.

KW - Benzo(a)pyrene

KW - Cell Cycle

KW - Cell Line

KW - Cell Survival

KW - DNA Adducts

KW - Gene Expression

KW - Gene Expression Profiling

KW - Humans

KW - Principal Component Analysis

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Time Factors

KW - Tumor Suppressor Protein p53

U2 - 10.1186/1471-2164-7-260

DO - 10.1186/1471-2164-7-260

M3 - Article

C2 - 17042939

VL - 7

JO - BMC Genomics

JF - BMC Genomics

SN - 1471-2164

M1 - 260

ER -