TY - JOUR
T1 - Transcriptional analysis of Rhazya stricta in response to jasmonic acid
AU - Hajrah, Nahid H.
AU - Rabah, Samar O.
AU - Alghamdi, Mohammed K.
AU - Atef, Ahmed
AU - Edris, Sherif
AU - Alhebshi, Alawiah M.
AU - Hassan, Sabah M.
AU - Alzahrani, Dhafer A.
AU - Bahieldin, Ahmed
AU - Mutwakil, Mohammed H. Z.
AU - Alqurashi, Yaser E.
AU - Al-Zahrani, Hassan S.
AU - Abo-Aba, Salah E. M.
AU - Jansen, Robert K.
AU - Sabir, Jamal S. M.
AU - Hall, Neil
AU - Kamli, Majid Rasool
N1 - Funding Information: This project was funded by the Deanship of Scientific Research (DSR) at King Abdulaziz University , Jeddah, under grant no. HiCi-35-130-36 . The authors, therefore, acknowledge with thanks the DSR for technical and financial support.
PY - 2021/3
Y1 - 2021/3
N2 - Background: Jasmonic acid (JA) is a signal transducer molecule that plays an important role in plant development and stress response; it can also efficiently stimulate secondary metabolism in plant cells. Results: RNA-Seq technology was applied to identify differentially expressed genes and study the time course of gene expression in Rhazya stricta in response to JA. Of more than 288 million total reads, approximately 27% were mapped to genes in the reference genome. Genes involved during the secondary metabolite pathways were up- or downregulated when treated with JA in R. stricta. Functional annotation and pathway analysis of all up- and downregulated genes identified many biological processes and molecular functions. Jasmonic acid biosynthetic, cell wall organization, and chlorophyll metabolic processes were upregulated at days 2, 6, and 12, respectively. Similarly, the molecular functions of calcium-transporting ATPase activity, ADP binding, and protein kinase activity were also upregulated at days 2, 6, and 12, respectively. Time-dependent transcriptional gene expression analysis showed that JA can induce signaling in the phenylpropanoid and aromatic acid pathways. These pathways are responsible for the production of secondary metabolites, which are essential for the development and environmental defense mechanism of R. stricta during stress conditions. Conclusions: Our results suggested that genes involved in flavonoid biosynthesis and aromatic acid synthesis pathways were upregulated during JA stress. However, monoterpenoid indole alkaloid (MIA) was unaffected by JA treatment. Hence, we can postulate that JA plays an important role in R. stricta during plant development and environmental stress conditions. How to cite: Hajrah, NH, Rabah SO, Alghamdi MK, et al. Transcriptional analysis of Rhazya stricta in response to jasmonic acid. Electron J Biotechnol 2021;50. https://doi.org/10.1016/j.ejbt.2021.01.004
AB - Background: Jasmonic acid (JA) is a signal transducer molecule that plays an important role in plant development and stress response; it can also efficiently stimulate secondary metabolism in plant cells. Results: RNA-Seq technology was applied to identify differentially expressed genes and study the time course of gene expression in Rhazya stricta in response to JA. Of more than 288 million total reads, approximately 27% were mapped to genes in the reference genome. Genes involved during the secondary metabolite pathways were up- or downregulated when treated with JA in R. stricta. Functional annotation and pathway analysis of all up- and downregulated genes identified many biological processes and molecular functions. Jasmonic acid biosynthetic, cell wall organization, and chlorophyll metabolic processes were upregulated at days 2, 6, and 12, respectively. Similarly, the molecular functions of calcium-transporting ATPase activity, ADP binding, and protein kinase activity were also upregulated at days 2, 6, and 12, respectively. Time-dependent transcriptional gene expression analysis showed that JA can induce signaling in the phenylpropanoid and aromatic acid pathways. These pathways are responsible for the production of secondary metabolites, which are essential for the development and environmental defense mechanism of R. stricta during stress conditions. Conclusions: Our results suggested that genes involved in flavonoid biosynthesis and aromatic acid synthesis pathways were upregulated during JA stress. However, monoterpenoid indole alkaloid (MIA) was unaffected by JA treatment. Hence, we can postulate that JA plays an important role in R. stricta during plant development and environmental stress conditions. How to cite: Hajrah, NH, Rabah SO, Alghamdi MK, et al. Transcriptional analysis of Rhazya stricta in response to jasmonic acid. Electron J Biotechnol 2021;50. https://doi.org/10.1016/j.ejbt.2021.01.004
KW - Environmental stress
KW - Flavonoid biosynthesis
KW - Gene expression
KW - Jasmonic acid
KW - Monoterpenoid indole alkaloid
KW - Phenylpropanoid aromatic acid pathway
KW - Rhazya stricta
KW - RNA sequencing
KW - Transcriptional gene expression analysis
KW - Transcriptome
UR - http://www.scopus.com/inward/record.url?scp=85101856105&partnerID=8YFLogxK
U2 - 10.1016/j.ejbt.2021.01.004
DO - 10.1016/j.ejbt.2021.01.004
M3 - Article
AN - SCOPUS:85101856105
VL - 50
SP - 68
EP - 76
JO - Electronic Journal of Biotechnology
JF - Electronic Journal of Biotechnology
SN - 0717-3458
ER -