TY - JOUR
T1 - Transcriptional attenuation control of the tylosin‐resistance gene tlrA in Streptomyces fradiae
AU - Kelemen, Gabriella H.
AU - Zalacain, Magdalena
AU - Culebras, Esther
AU - Seno, Eugene T.
AU - Cundliffe, Eric
PY - 1994/11
Y1 - 1994/11
N2 - The tylosin producer Streptomyces fradiae contains four known resistance genes, two of which (tlrA and tlrD) encode methyltransferases that act on ribosomal RNA at a common site. Expression of tlrA is regulated via transcriptional attenuation. A short transcript, only 411 nucleotides long, terminates 27 nucleotides into the methylase‐coding sequence in the uninduced state. Induction of tlrA is proposed to involve a ribosome‐mediated conformational change within the mRNA leader that allows transcription to continue beyond the attenuation site, resulting in a transcript about 1450 nucleotides long. Transplantation of tlrD and/or tlrA into Streptomyces albus revealed that the induction specificity of tlrA depends upon the state of the ribosomes and is significantly altered in strains also expressing tlrD.
AB - The tylosin producer Streptomyces fradiae contains four known resistance genes, two of which (tlrA and tlrD) encode methyltransferases that act on ribosomal RNA at a common site. Expression of tlrA is regulated via transcriptional attenuation. A short transcript, only 411 nucleotides long, terminates 27 nucleotides into the methylase‐coding sequence in the uninduced state. Induction of tlrA is proposed to involve a ribosome‐mediated conformational change within the mRNA leader that allows transcription to continue beyond the attenuation site, resulting in a transcript about 1450 nucleotides long. Transplantation of tlrD and/or tlrA into Streptomyces albus revealed that the induction specificity of tlrA depends upon the state of the ribosomes and is significantly altered in strains also expressing tlrD.
UR - http://www.scopus.com/inward/record.url?scp=0028171565&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2958.1994.tb01319.x
DO - 10.1111/j.1365-2958.1994.tb01319.x
M3 - Article
C2 - 7534372
AN - SCOPUS:0028171565
SN - 0950-382X
VL - 14
SP - 833
EP - 842
JO - Molecular Microbiology
JF - Molecular Microbiology
IS - 4
ER -