Transcriptional attenuation control of the tylosin‐resistance gene tlrA in Streptomyces fradiae

Gabriella H. Kelemen, Magdalena Zalacain, Esther Culebras, Eugene T. Seno, Eric Cundliffe

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The tylosin producer Streptomyces fradiae contains four known resistance genes, two of which (tlrA and tlrD) encode methyltransferases that act on ribosomal RNA at a common site. Expression of tlrA is regulated via transcriptional attenuation. A short transcript, only 411 nucleotides long, terminates 27 nucleotides into the methylase‐coding sequence in the uninduced state. Induction of tlrA is proposed to involve a ribosome‐mediated conformational change within the mRNA leader that allows transcription to continue beyond the attenuation site, resulting in a transcript about 1450 nucleotides long. Transplantation of tlrD and/or tlrA into Streptomyces albus revealed that the induction specificity of tlrA depends upon the state of the ribosomes and is significantly altered in strains also expressing tlrD.

Original languageEnglish
Pages (from-to)833-842
Number of pages10
JournalMolecular Microbiology
Issue number4
Publication statusPublished - Nov 1994

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