Projects per year
Arabidopsis thaliana accessions are universally resistant at the adult leaf stage to white rust (Albugo candida) races that infect the crop species Brassica juncea and Brassica oleracea. We used transgressive segregation in recombinant inbred lines to test if this apparent species-wide (nonhost) resistance in A. thaliana is due to natural pyramiding of multiple Resistance (R) genes. We screened 593 inbred lines from an Arabidopsis multiparent advanced generation intercross (MAGIC) mapping population, derived from 19 resistant parental accessions, and identified two transgressive segregants that are susceptible to the pathogen. These were crossed to each MAGIC parent, and analysis of resulting F 2 progeny followed by positional cloning showed that resistance to an isolate of A. candida race 2 (Ac2V) can be explained in each accession by at least one of four genes encoding nucleotide-binding, leucine-rich repeat (NLR) immune receptors. An additional gene was identified that confers resistance to an isolate of A. candida race 9 (AcBoT) that infects B. oleracea. Thus, effector-triggered immunity conferred by distinct NLR-encoding genes in multiple A. thaliana accessions provides species-wide resistance to these crop pathogens.
|Number of pages||7|
|Journal||Proceedings of the National Academy of Sciences of the United States of America|
|Early online date||28 Jan 2019|
|Publication status||Published - 12 Feb 2019|
- Albugo candida
- Arabidopsis thaliana
- Nonhost resistance
- 2 Finished
1/10/17 → 30/09/21
Gene-for-gene coevolution between Albugo candida and Arabidopsis; mining non-host resistance genes for white rust control in Brassicaceae crops
Jones, J., Cevik, V., Etherington, G. & Smoker, M.
1/12/14 → 31/01/18