Transgressive segregation reveals mechanisms of Arabidopsis immunity to Brassica-infecting races of white rust (Albugo candida)

Volkan Cevik, Freddy Boutrot, Wiebke Apel, Alexandre Robert-Seilaniantz, Oliver J. Furzer, Amey Redkar, Baptiste Castel, Paula X. Kover, David C. Prince, Eric B. Holub, Jonathan D.G. Jones

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Arabidopsis thaliana accessions are universally resistant at the adult leaf stage to white rust (Albugo candida) races that infect the crop species Brassica juncea and Brassica oleracea. We used transgressive segregation in recombinant inbred lines to test if this apparent species-wide (nonhost) resistance in A. thaliana is due to natural pyramiding of multiple Resistance (R) genes. We screened 593 inbred lines from an Arabidopsis multiparent advanced generation intercross (MAGIC) mapping population, derived from 19 resistant parental accessions, and identified two transgressive segregants that are susceptible to the pathogen. These were crossed to each MAGIC parent, and analysis of resulting F 2 progeny followed by positional cloning showed that resistance to an isolate of A. candida race 2 (Ac2V) can be explained in each accession by at least one of four genes encoding nucleotide-binding, leucine-rich repeat (NLR) immune receptors. An additional gene was identified that confers resistance to an isolate of A. candida race 9 (AcBoT) that infects B. oleracea. Thus, effector-triggered immunity conferred by distinct NLR-encoding genes in multiple A. thaliana accessions provides species-wide resistance to these crop pathogens.

Original languageEnglish
Pages (from-to)2767-2773
Number of pages7
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number7
Early online date28 Jan 2019
Publication statusPublished - 12 Feb 2019


  • Albugo candida
  • Arabidopsis thaliana
  • Brassicaceae
  • Nonhost resistance
  • Oomycete

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