Translation of cyclin mRNA is necessary for extracts of activated xenopus eggs to enter mitosis

Jeremy Minshull, J. Julian Blow, Tim Hunt

Research output: Contribution to journalArticlepeer-review

360 Citations (Scopus)


The cyclins are a family of proteins encoded by maternal mRNA. Cyclin polypeptides accumulate during interphase and are destroyed during mitosis at about the time of entry into anaphase. We show here that Xenopus oocytes contain mRNAs encoding two cyclins that are major translation products in a cell-free extract from activated eggs. Cutting these mRNAs with antisense oligonucleotides and endogenous RNAase H blocks entry into mitosis in a cell-free egg extract. The extracts can enter mitosis if either of the cyclin mRNAs is left intact. We conclude that the synthesis of these cyclins is necessary for mitotic cell cycles in cleaving Xenopus embryos.
Original languageEnglish
Pages (from-to)947-956
Number of pages10
Issue number6
Publication statusPublished - 24 Mar 1989

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