Background: Methods for rapid assessment of interactions of small molecules with membrane proteins in detergent are lacking.
Results: Thermostability measurements of mitochondrial transporters display informative trends about detergent, lipid, substrate, and inhibitor interactions.
Conclusion: Mechanistic insights are obtained by studying the thermostability of mitochondrial transporters.
Significance: Information about the nature of compound interactions with membrane proteins can be obtained rapidly.
|Number of pages||12|
|Journal||Journal of Biological Chemistry|
|Early online date||4 Feb 2015|
|Publication status||Published - 27 Mar 2015|
- Membrane Protein
- Mitochondrial Transport
- Protein Stability
- Uncoupling Protein
- Adenine Nucleotide Translocase
- Detergent Micelle
- Differential Scanning Fluorimetry
- Thermofluor Assay
Person: Research Group Member, Academic, Teaching & Research