Abstract
Background: Methods for rapid assessment of interactions of small molecules with membrane proteins in detergent are lacking.
Results: Thermostability measurements of mitochondrial transporters display informative trends about detergent, lipid, substrate, and inhibitor interactions.
Conclusion: Mechanistic insights are obtained by studying the thermostability of mitochondrial transporters.
Significance: Information about the nature of compound interactions with membrane proteins can be obtained rapidly.
Original language | English |
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Pages (from-to) | 8206-8217 |
Number of pages | 12 |
Journal | Journal of Biological Chemistry |
Volume | 290 |
Issue number | 13 |
Early online date | 4 Feb 2015 |
DOIs | |
Publication status | Published - 27 Mar 2015 |
Keywords
- Membrane Protein
- Mitochondrial Transport
- Protein Stability
- Transporter
- Uncoupling Protein
- Adenine Nucleotide Translocase
- Detergent Micelle
- Differential Scanning Fluorimetry
- Thermofluor Assay
Profiles
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Paul Crichton
Person: Research Group Member, Research Centre Member, Academic, Teaching & Research