Trends in thermostability provide information on the nature of substrate, inhibitor, and lipid interactions with mitochondrial carriers

Paul G. Crichton; Yang Lee; Jonathan J. Ruprecht; Elizabeth Cerson; Chancievan Thangaratnarajah; Martin S. King; Edmund R. S. Kunji

doi:10.1074/jbc.M114.616607

Trends in thermostability provide information on the nature of substrate, inhibitor, and lipid interactions with mitochondrial carriers

Paul G. Crichton
, Yang Lee
, Jonathan J. Ruprecht
, Elizabeth Cerson
, Chancievan Thangaratnarajah
, Martin S. King
, Edmund R. S. Kunji

Research output: Contribution to journal › Article › peer-review

70 Citations (Scopus)

19 Downloads (Pure)

Abstract

Background: Methods for rapid assessment of interactions of small molecules with membrane proteins in detergent are lacking.

Results: Thermostability measurements of mitochondrial transporters display informative trends about detergent, lipid, substrate, and inhibitor interactions.

Conclusion: Mechanistic insights are obtained by studying the thermostability of mitochondrial transporters.

Significance: Information about the nature of compound interactions with membrane proteins can be obtained rapidly.

Original language	English
Pages (from-to)	8206-8217
Number of pages	12
Journal	Journal of Biological Chemistry
Volume	290
Issue number	13
Early online date	4 Feb 2015
DOIs	https://doi.org/10.1074/jbc.M114.616607
Publication status	Published - 27 Mar 2015

Keywords

Membrane Protein
Mitochondrial Transport
Protein Stability
Transporter
Uncoupling Protein
Adenine Nucleotide Translocase
Detergent Micelle
Differential Scanning Fluorimetry
Thermofluor Assay

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10.1074/jbc.M114.616607Licence: CC BY

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http://www.jbc.org/content/290/13/8206Licence: CC BY

Paul Crichton
- P.Crichtonuea.acuk
- Norwich Medical School - Professor
- Metabolic Health - Member
- Cardiovascular and Metabolic Health - Member
Person: Research Group Member, Research Centre Member, Academic, Teaching and Research

Cite this

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title = "Trends in thermostability provide information on the nature of substrate, inhibitor, and lipid interactions with mitochondrial carriers",

abstract = "Background: Methods for rapid assessment of interactions of small molecules with membrane proteins in detergent are lacking. Results: Thermostability measurements of mitochondrial transporters display informative trends about detergent, lipid, substrate, and inhibitor interactions. Conclusion: Mechanistic insights are obtained by studying the thermostability of mitochondrial transporters. Significance: Information about the nature of compound interactions with membrane proteins can be obtained rapidly.",

keywords = "Membrane Protein, Mitochondrial Transport, Protein Stability, Transporter, Uncoupling Protein, Adenine Nucleotide Translocase, Detergent Micelle, Differential Scanning Fluorimetry, Thermofluor Assay",

author = "Crichton, \{Paul G.\} and Yang Lee and Ruprecht, \{Jonathan J.\} and Elizabeth Cerson and Chancievan Thangaratnarajah and King, \{Martin S.\} and Kunji, \{Edmund R. S.\}",

year = "2015",

month = mar,

day = "27",

doi = "10.1074/jbc.M114.616607",

language = "English",

volume = "290",

pages = "8206--8217",

journal = "Journal of Biological Chemistry",

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T1 - Trends in thermostability provide information on the nature of substrate, inhibitor, and lipid interactions with mitochondrial carriers

AU - Crichton, Paul G.

AU - Lee, Yang

AU - Ruprecht, Jonathan J.

AU - Cerson, Elizabeth

AU - Thangaratnarajah, Chancievan

AU - King, Martin S.

AU - Kunji, Edmund R. S.

PY - 2015/3/27

Y1 - 2015/3/27

N2 - Background: Methods for rapid assessment of interactions of small molecules with membrane proteins in detergent are lacking. Results: Thermostability measurements of mitochondrial transporters display informative trends about detergent, lipid, substrate, and inhibitor interactions. Conclusion: Mechanistic insights are obtained by studying the thermostability of mitochondrial transporters. Significance: Information about the nature of compound interactions with membrane proteins can be obtained rapidly.

AB - Background: Methods for rapid assessment of interactions of small molecules with membrane proteins in detergent are lacking. Results: Thermostability measurements of mitochondrial transporters display informative trends about detergent, lipid, substrate, and inhibitor interactions. Conclusion: Mechanistic insights are obtained by studying the thermostability of mitochondrial transporters. Significance: Information about the nature of compound interactions with membrane proteins can be obtained rapidly.

KW - Membrane Protein

KW - Mitochondrial Transport

KW - Protein Stability

KW - Transporter

KW - Uncoupling Protein

KW - Adenine Nucleotide Translocase

KW - Detergent Micelle

KW - Differential Scanning Fluorimetry

KW - Thermofluor Assay

U2 - 10.1074/jbc.M114.616607

DO - 10.1074/jbc.M114.616607

M3 - Article

C2 - 25653283

AN - SCOPUS:84925798847

SN - 0021-9258

VL - 290

SP - 8206

EP - 8217

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 13

ER -

Trends in thermostability provide information on the nature of substrate, inhibitor, and lipid interactions with mitochondrial carriers

Abstract

Keywords

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Paul Crichton

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