Ultrafast dynamics of protein proton transfer on short hydrogen bond potential energy surfaces: S65T/H148D GFP

Minako Kondo; Ismael Heisler; Deborah Stoner-Ma; Peter J. Tonge; Steve Meech

doi:10.1021/ja907690g

Ultrafast dynamics of protein proton transfer on short hydrogen bond potential energy surfaces: S65T/H148D GFP

Minako Kondo, Ismael Heisler, Deborah Stoner-Ma, Peter J. Tonge, Steve Meech

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

Ultrafast proton transfer dynamics on a short H-bond in a protein were studied through the time-resolved fluorescence of the S65T/H148D green fluorescent protein (GFP) mutant. In response to the change in chromophore pK_a upon excitation, the donor-proton-acceptor structure evolves on a sub-100 fs time scale, followed by picosecond time scale vibrational cooling and host structure reorganization.

Original language	English
Pages (from-to)	1452-1453
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	132
Issue number	5
DOIs	https://doi.org/10.1021/ja907690g
Publication status	Published - 2010

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10.1021/ja907690g

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title = "Ultrafast dynamics of protein proton transfer on short hydrogen bond potential energy surfaces: S65T/H148D GFP",

abstract = "Ultrafast proton transfer dynamics on a short H-bond in a protein were studied through the time-resolved fluorescence of the S65T/H148D green fluorescent protein (GFP) mutant. In response to the change in chromophore pKa upon excitation, the donor-proton-acceptor structure evolves on a sub-100 fs time scale, followed by picosecond time scale vibrational cooling and host structure reorganization.",

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AU - Kondo, Minako

AU - Heisler, Ismael

AU - Stoner-Ma, Deborah

AU - Tonge, Peter J.

AU - Meech, Steve

PY - 2010

Y1 - 2010

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AB - Ultrafast proton transfer dynamics on a short H-bond in a protein were studied through the time-resolved fluorescence of the S65T/H148D green fluorescent protein (GFP) mutant. In response to the change in chromophore pKa upon excitation, the donor-proton-acceptor structure evolves on a sub-100 fs time scale, followed by picosecond time scale vibrational cooling and host structure reorganization.

U2 - 10.1021/ja907690g

DO - 10.1021/ja907690g

M3 - Article

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EP - 1453

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

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