Ultrafast Dynamics of Protein Proton Transfer on Short Hydrogen Bond Potential Energy Surfaces: S65T/H148D GFP.

Minako Kondo, Ismael Heisler, Deborah Stoner-Ma, Peter J. Tonge, Steve Meech

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38 Citations (Scopus)

Abstract

Ultrafast proton transfer dynamics on a short H-bond in a protein were studied through the time-resolved fluorescence of the S65T/H148D green fluorescent protein (GFP) mutant. In response to the change in chromophore pKa upon excitation, the donor-proton-acceptor structure evolves on a sub-100 fs time scale, followed by picosecond time scale vibrational cooling and host structure reorganization.
Original languageEnglish
Pages (from-to)1452-1453
Number of pages2
JournalJournal of the American Chemical Society
Volume132
Issue number5
DOIs
Publication statusPublished - 2010

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