Ultrafast studies of the photophysics of cis and trans states of the green fluorescent protein chromophore

Kiri Addison; Jamie Conyard; Tara Dixon; Philip C. Bulman Page; Kyril M. Solntsev; Stephen R. Meech

doi:10.1021/jz3008408

Ultrafast studies of the photophysics of cis and trans states of the green fluorescent protein chromophore

Kiri Addison, Jamie Conyard, Tara Dixon, Philip C. Bulman Page, Kyril M. Solntsev, Stephen R. Meech

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Abstract

Cis–trans photoisomerization is proposed as a key process in the photoswitching of some photoactivatable fluorescent proteins. Here we present ultrafast fluorescence measurements of the model GFP chromophore (HBDI) in the cis state and in a mixture of the cis and trans states. Our results demonstrate that the mean lifetimes of the cis and trans states are remarkably similar. Therefore, the specific isomer of the chromophore cannot be solely responsible for the different photophysics of the bright and dark states of photoactive proteins, which must therefore be due to differential interactions between the different isomers of the chromophore and the protein.

Original language	English
Pages (from-to)	2298-2302
Number of pages	5
Journal	The Journal of Physical Chemistry Letters
Volume	3
Issue number	16
DOIs	https://doi.org/10.1021/jz3008408
Publication status	Published - 2012

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10.1021/jz3008408

Cite this

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abstract = "Cis–trans photoisomerization is proposed as a key process in the photoswitching of some photoactivatable fluorescent proteins. Here we present ultrafast fluorescence measurements of the model GFP chromophore (HBDI) in the cis state and in a mixture of the cis and trans states. Our results demonstrate that the mean lifetimes of the cis and trans states are remarkably similar. Therefore, the specific isomer of the chromophore cannot be solely responsible for the different photophysics of the bright and dark states of photoactive proteins, which must therefore be due to differential interactions between the different isomers of the chromophore and the protein.",

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AU - Addison, Kiri

AU - Conyard, Jamie

AU - Dixon, Tara

AU - Bulman Page, Philip C.

AU - Solntsev, Kyril M.

AU - Meech, Stephen R.

PY - 2012

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N2 - Cis–trans photoisomerization is proposed as a key process in the photoswitching of some photoactivatable fluorescent proteins. Here we present ultrafast fluorescence measurements of the model GFP chromophore (HBDI) in the cis state and in a mixture of the cis and trans states. Our results demonstrate that the mean lifetimes of the cis and trans states are remarkably similar. Therefore, the specific isomer of the chromophore cannot be solely responsible for the different photophysics of the bright and dark states of photoactive proteins, which must therefore be due to differential interactions between the different isomers of the chromophore and the protein.

AB - Cis–trans photoisomerization is proposed as a key process in the photoswitching of some photoactivatable fluorescent proteins. Here we present ultrafast fluorescence measurements of the model GFP chromophore (HBDI) in the cis state and in a mixture of the cis and trans states. Our results demonstrate that the mean lifetimes of the cis and trans states are remarkably similar. Therefore, the specific isomer of the chromophore cannot be solely responsible for the different photophysics of the bright and dark states of photoactive proteins, which must therefore be due to differential interactions between the different isomers of the chromophore and the protein.

U2 - 10.1021/jz3008408

DO - 10.1021/jz3008408

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