UPLC-MS/MS Determination of Deuterated 25-Hydroxyvitamin D (d3-25OHD3) and Other Vitamin D Metabolites for the Measurement of 25OHD Half-Life

Shima Assar; Inez Schoenmakers; Albert Koulman; Ann Prentice; Kerry S Jones

doi:10.1007/978-1-4939-6730-8_22

UPLC-MS/MS Determination of Deuterated 25-Hydroxyvitamin D (d3-25OHD3) and Other Vitamin D Metabolites for the Measurement of 25OHD Half-Life

Shima Assar, Inez Schoenmakers, Albert Koulman, Ann Prentice, Kerry S Jones

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

1 Citation (Scopus)

Abstract

Plasma 25-hydroxyvitamin D (25OHD) half-life (25OHDt 1/2) is a dynamic marker of vitamin D metabolism that can be used to assess vitamin D expenditure and help inform vitamin D requirements. Our group recently established an approach to determine the 25OHDt 1/2 as an alternative biomarker of 25OHD expenditure in humans. The approach uses a small oral dose of stable isotope labeled 25OHD3 [3-2H-25OHD3 (6,19,19-d3)] (d3-25OHD3) (tracer), which is distinguishable from endogenous 25OHD by liquid chromatography tandem-mass spectrometry (LC-MS/MS). We report here the method, which relies on protein precipitation, purification with solid phase extraction, derivatization with 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD), and determination of the compounds by isotope-dilution UPLC-MS/MS. The method proved to be rapid and sensitive (LOQ 0.2 nmol/L) for the quantification of this tracer as well as the other vitamin D metabolites: 25OHD3, 25OHD2, and 24,25(OH)2D3 in human plasma.

Original language	English
Title of host publication	Multiplex Biomarker Techniques
Subtitle of host publication	Methods and Applications
Editors	Paul C. Guest
Publisher	Springer
Pages	257-265
Number of pages	9
ISBN (Electronic)	978-1-4939-6730-8
ISBN (Print)	978-1-4939-6729-2
DOIs	https://doi.org/10.1007/978-1-4939-6730-8_22
Publication status	Published - 29 Nov 2016

Publication series

Name	Methods in Molecular Biology
Publisher	Springer New York
Volume	1546
ISSN (Print)	1064-3745

Access to Document

10.1007/978-1-4939-6730-8_22

Cite this

Assar, S., Schoenmakers, I., Koulman, A., Prentice, A., & Jones, K. S. (2016). UPLC-MS/MS Determination of Deuterated 25-Hydroxyvitamin D (d3-25OHD3) and Other Vitamin D Metabolites for the Measurement of 25OHD Half-Life. In P. C. Guest (Ed.), Multiplex Biomarker Techniques: Methods and Applications (pp. 257-265). (Methods in Molecular Biology; Vol. 1546). Springer. https://doi.org/10.1007/978-1-4939-6730-8_22

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abstract = "Plasma 25-hydroxyvitamin D (25OHD) half-life (25OHDt 1/2) is a dynamic marker of vitamin D metabolism that can be used to assess vitamin D expenditure and help inform vitamin D requirements. Our group recently established an approach to determine the 25OHDt 1/2 as an alternative biomarker of 25OHD expenditure in humans. The approach uses a small oral dose of stable isotope labeled 25OHD3 [3-2H-25OHD3 (6,19,19-d3)] (d3-25OHD3) (tracer), which is distinguishable from endogenous 25OHD by liquid chromatography tandem-mass spectrometry (LC-MS/MS). We report here the method, which relies on protein precipitation, purification with solid phase extraction, derivatization with 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD), and determination of the compounds by isotope-dilution UPLC-MS/MS. The method proved to be rapid and sensitive (LOQ 0.2 nmol/L) for the quantification of this tracer as well as the other vitamin D metabolites: 25OHD3, 25OHD2, and 24,25(OH)2D3 in human plasma.",

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Assar, S, Schoenmakers, I, Koulman, A, Prentice, A & Jones, KS 2016, UPLC-MS/MS Determination of Deuterated 25-Hydroxyvitamin D (d3-25OHD3) and Other Vitamin D Metabolites for the Measurement of 25OHD Half-Life. in PC Guest (ed.), Multiplex Biomarker Techniques: Methods and Applications. Methods in Molecular Biology, vol. 1546, Springer, pp. 257-265. https://doi.org/10.1007/978-1-4939-6730-8_22

UPLC-MS/MS Determination of Deuterated 25-Hydroxyvitamin D (d3-25OHD3) and Other Vitamin D Metabolites for the Measurement of 25OHD Half-Life. / Assar, Shima; Schoenmakers, Inez; Koulman, Albert et al.

Multiplex Biomarker Techniques: Methods and Applications. ed. / Paul C. Guest. Springer, 2016. p. 257-265 (Methods in Molecular Biology; Vol. 1546).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

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AU - Assar, Shima

AU - Schoenmakers, Inez

AU - Koulman, Albert

AU - Prentice, Ann

AU - Jones, Kerry S

PY - 2016/11/29

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N2 - Plasma 25-hydroxyvitamin D (25OHD) half-life (25OHDt 1/2) is a dynamic marker of vitamin D metabolism that can be used to assess vitamin D expenditure and help inform vitamin D requirements. Our group recently established an approach to determine the 25OHDt 1/2 as an alternative biomarker of 25OHD expenditure in humans. The approach uses a small oral dose of stable isotope labeled 25OHD3 [3-2H-25OHD3 (6,19,19-d3)] (d3-25OHD3) (tracer), which is distinguishable from endogenous 25OHD by liquid chromatography tandem-mass spectrometry (LC-MS/MS). We report here the method, which relies on protein precipitation, purification with solid phase extraction, derivatization with 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD), and determination of the compounds by isotope-dilution UPLC-MS/MS. The method proved to be rapid and sensitive (LOQ 0.2 nmol/L) for the quantification of this tracer as well as the other vitamin D metabolites: 25OHD3, 25OHD2, and 24,25(OH)2D3 in human plasma.

AB - Plasma 25-hydroxyvitamin D (25OHD) half-life (25OHDt 1/2) is a dynamic marker of vitamin D metabolism that can be used to assess vitamin D expenditure and help inform vitamin D requirements. Our group recently established an approach to determine the 25OHDt 1/2 as an alternative biomarker of 25OHD expenditure in humans. The approach uses a small oral dose of stable isotope labeled 25OHD3 [3-2H-25OHD3 (6,19,19-d3)] (d3-25OHD3) (tracer), which is distinguishable from endogenous 25OHD by liquid chromatography tandem-mass spectrometry (LC-MS/MS). We report here the method, which relies on protein precipitation, purification with solid phase extraction, derivatization with 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD), and determination of the compounds by isotope-dilution UPLC-MS/MS. The method proved to be rapid and sensitive (LOQ 0.2 nmol/L) for the quantification of this tracer as well as the other vitamin D metabolites: 25OHD3, 25OHD2, and 24,25(OH)2D3 in human plasma.

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M3 - Chapter (peer-reviewed)

SN - 978-1-4939-6729-2

T3 - Methods in Molecular Biology

SP - 257

EP - 265

BT - Multiplex Biomarker Techniques

A2 - Guest, Paul C.

PB - Springer

ER -

Assar S, Schoenmakers I, Koulman A, Prentice A, Jones KS. UPLC-MS/MS Determination of Deuterated 25-Hydroxyvitamin D (d3-25OHD3) and Other Vitamin D Metabolites for the Measurement of 25OHD Half-Life. In Guest PC, editor, Multiplex Biomarker Techniques: Methods and Applications. Springer. 2016. p. 257-265. (Methods in Molecular Biology). doi: 10.1007/978-1-4939-6730-8_22