We report the application of "click" chemistry for the synthesis of hybrid calixarenes appended on the upper rim with carbohydrate and N,C-protected α-amino acids. The chemoselective N- or C-deprotection of the α-amino acids and their subsequent transformation into dipeptides is described. The first example of a chemo-enzymatic synthesis on upper rim derived calixarenes using trans-sialidase affords sialylated lactose calixarenes. Our innovative chemo-enzymatic process paves the way for further applications. © 2007 American Chemical Society.