TY - JOUR
T1 - Use of a probiotic to control lactococcosis and streptococcosis in rainbow trout, Oncorhynchus mykiss (Walbaum)
AU - Brunt, J.
AU - Austin, B.
PY - 2005/12
Y1 - 2005/12
N2 - From a comparison of 125 bacterial isolates recovered from the digestive tract of rainbow trout, Oncorhynchus mykiss, and carp, Cyprinus sp., a culture was obtained which was effective at preventing clinical disease caused by Lactococcus garvieae and Streptococcus iniae when used as a feed additive. The culture, Aeromonas sobria GC2, was incorporated into the feed and fed to rainbow trout (average weight = 20 g) for 14 days at a dose equivalent to 5 × 107 cells g−1 of feed. Whereas the untreated controls experienced losses of 75–100% when challenged intraperitoneally with L. garvieae and S. iniae, the probiotic-treated groups remained healthy with total mortalities of only 0–6%. Formalized and sonicated preparations of GC2 and cell-free supernatant fared less well. The mode of action reflected stimulation of innate immunity, namely an increased number of leucocytes and enhanced phagocytic and respiratory burst activity.
AB - From a comparison of 125 bacterial isolates recovered from the digestive tract of rainbow trout, Oncorhynchus mykiss, and carp, Cyprinus sp., a culture was obtained which was effective at preventing clinical disease caused by Lactococcus garvieae and Streptococcus iniae when used as a feed additive. The culture, Aeromonas sobria GC2, was incorporated into the feed and fed to rainbow trout (average weight = 20 g) for 14 days at a dose equivalent to 5 × 107 cells g−1 of feed. Whereas the untreated controls experienced losses of 75–100% when challenged intraperitoneally with L. garvieae and S. iniae, the probiotic-treated groups remained healthy with total mortalities of only 0–6%. Formalized and sonicated preparations of GC2 and cell-free supernatant fared less well. The mode of action reflected stimulation of innate immunity, namely an increased number of leucocytes and enhanced phagocytic and respiratory burst activity.
UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-33745309891&partnerID=MN8TOARS
U2 - 10.1111/j.1365-2761.2005.00672.x
DO - 10.1111/j.1365-2761.2005.00672.x
M3 - Article
SN - 0140-7775
VL - 28
SP - 693
EP - 701
JO - Journal of Fish Diseases
JF - Journal of Fish Diseases
IS - 12
ER -