Projects per year
The tomato PROCERA gene encodes a DELLA protein, and loss-of-function mutations derepress growth. We used CRISPR/Cas9 and a single guide RNAs (sgRNA) to target mutations to the PROCERA DELLA domain, and recovered several loss-of-function mutations and a dominant dwarf mutation that carries a deletion of one amino acid in the DELLA domain. This is the first report of a dominant dwarf PROCERA allele. This allele retains partial responsiveness to exogenously applied gibberellin. Heterozygotes show an intermediate phenotype at the seedling stage, but adult heterozygotes are as dwarfed as homozygotes.
- gibberellic acid
- 1 Finished
Controlling important diseases in potato by cloning and engineering functional NB-LRR-type resistance genes
Hein, I., Jones, J., Smoker, M., Smoker, M. & Perkins, S.
1/04/14 → 31/03/17