Using CRISPR/Cas9 genome editing in tomato to create a gibberellin-responsive dominant dwarf DELLA allele

Laurence Tomlinson, Ying Yang, Ryan Emenecker, Matthew Smoker, Jodie Taylor, Sara Perkins, Justine Smith, Dan MacLean, Neil E. Olszewski, Jonathan D. G. Jones

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36 Citations (Scopus)
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Abstract

The tomato PROCERA gene encodes a DELLA protein, and loss-of-function mutations derepress growth. We used CRISPR/Cas9 and a single guide RNAs (sgRNA) to target mutations to the PROCERA DELLA domain, and recovered several loss-of-function mutations and a dominant dwarf mutation that carries a deletion of one amino acid in the DELLA domain. This is the first report of a dominant dwarf PROCERA allele. This allele retains partial responsiveness to exogenously applied gibberellin. Heterozygotes show an intermediate phenotype at the seedling stage, but adult heterozygotes are as dwarfed as homozygotes.

Original languageEnglish
Pages (from-to)132-140
Number of pages9
JournalPlant Biotechnology Journal
Volume17
Issue number1
Early online date24 May 2018
DOIs
Publication statusPublished - Jan 2019

Keywords

  • CRISPR/Cas9
  • DELLA
  • dwarf
  • gibberellic acid
  • PROCERA
  • slender

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