Using CRISPR/Cas9 genome editing in tomato to create a gibberellin-responsive dominant dwarf DELLA allele

Laurence Tomlinson; Ying Yang; Ryan Emenecker; Matthew Smoker; Jodie Taylor; Sara Perkins; Justine Smith; Dan MacLean; Neil E. Olszewski; Jonathan D. G. Jones

doi:10.1111/pbi.12952

Using CRISPR/Cas9 genome editing in tomato to create a gibberellin-responsive dominant dwarf DELLA allele

Laurence Tomlinson, Ying Yang, Ryan Emenecker, Matthew Smoker, Jodie Taylor, Sara Perkins, Justine Smith, Dan MacLean, Neil E. Olszewski, Jonathan D. G. Jones

Research output: Contribution to journal › Article › peer-review

92 Citations (Scopus)

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Abstract

The tomato PROCERA gene encodes a DELLA protein, and loss-of-function mutations derepress growth. We used CRISPR/Cas9 and a single guide RNAs (sgRNA) to target mutations to the PROCERA DELLA domain, and recovered several loss-of-function mutations and a dominant dwarf mutation that carries a deletion of one amino acid in the DELLA domain. This is the first report of a dominant dwarf PROCERA allele. This allele retains partial responsiveness to exogenously applied gibberellin. Heterozygotes show an intermediate phenotype at the seedling stage, but adult heterozygotes are as dwarfed as homozygotes.

Original language	English
Pages (from-to)	132-140
Number of pages	9
Journal	Plant Biotechnology Journal
Volume	17
Issue number	1
Early online date	24 May 2018
DOIs	https://doi.org/10.1111/pbi.12952
Publication status	Published - Jan 2019

Keywords

CRISPR/Cas9
DELLA
dwarf
gibberellic acid
PROCERA
slender

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10.1111/pbi.12952Licence: CC BY

pbi.12952Final published version, 974 KBLicence: CC BY

Cite this

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title = "Using CRISPR/Cas9 genome editing in tomato to create a gibberellin-responsive dominant dwarf DELLA allele",

abstract = "The tomato PROCERA gene encodes a DELLA protein, and loss-of-function mutations derepress growth. We used CRISPR/Cas9 and a single guide RNAs (sgRNA) to target mutations to the PROCERA DELLA domain, and recovered several loss-of-function mutations and a dominant dwarf mutation that carries a deletion of one amino acid in the DELLA domain. This is the first report of a dominant dwarf PROCERA allele. This allele retains partial responsiveness to exogenously applied gibberellin. Heterozygotes show an intermediate phenotype at the seedling stage, but adult heterozygotes are as dwarfed as homozygotes.",

keywords = "CRISPR/Cas9, DELLA, dwarf, gibberellic acid, PROCERA, slender",

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AU - Tomlinson, Laurence

AU - Yang, Ying

AU - Emenecker, Ryan

AU - Smoker, Matthew

AU - Taylor, Jodie

AU - Perkins, Sara

AU - Smith, Justine

AU - MacLean, Dan

AU - Olszewski, Neil E.

AU - Jones, Jonathan D. G.

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AB - The tomato PROCERA gene encodes a DELLA protein, and loss-of-function mutations derepress growth. We used CRISPR/Cas9 and a single guide RNAs (sgRNA) to target mutations to the PROCERA DELLA domain, and recovered several loss-of-function mutations and a dominant dwarf mutation that carries a deletion of one amino acid in the DELLA domain. This is the first report of a dominant dwarf PROCERA allele. This allele retains partial responsiveness to exogenously applied gibberellin. Heterozygotes show an intermediate phenotype at the seedling stage, but adult heterozygotes are as dwarfed as homozygotes.

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Using CRISPR/Cas9 genome editing in tomato to create a gibberellin-responsive dominant dwarf DELLA allele

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Keywords

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Controlling important diseases in potato by cloning and engineering functional NB-LRR-type resistance genes

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Using CRISPR/Cas9 genome editing in tomato to create a gibberellin-responsive dominant dwarf DELLA allele

Abstract

Keywords

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Controlling important diseases in potato by cloning and engineering functional NB-LRR-type resistance genes

Cite this