Abstract
The demand for measurement of vitamin D metabolites for clinical diagnosis and to advance our understanding of the role of vitamin D in human health has significantly increased in the last decade. New developments in technologies employed have enabled the separation and quantification of additional metabolites and interferences. Also, developments of immunoassays have changed the landscape. Programmes and materials for assay standardisation, harmonisation and the expansion of the vitamin D external quality assurance scheme (DEQAS) with the provision of target values as measured by a reference measurement procedure have improved standardisation, quality assurance and comparability of measurements. In this article, we describe developments in the measurement of the commonly analysed vitamin D metabolites in clinical and research practice. We describe current analytical approaches, discuss differences between assays, their origin, and how these may be influenced by physiological and experimental conditions. The value of measuring metabolites beyond 25 hydroxyvitamin D (25(OH)D), the marker of vitamin D status, in routine clinical practice is not yet confirmed. Here we provide an overview of the value and application of the measurement of 1,25 dihydroxyvitamin D, 24,25 dihydroxyvitamin D and free 25OHD in the diagnosis of patients with abnormalities in vitamin D metabolism and for research purposes.
Original language | English |
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Pages (from-to) | 3-13 |
Number of pages | 11 |
Journal | Calcified Tissue International |
Volume | 106 |
Issue number | 1 |
Early online date | 18 Nov 2019 |
DOIs | |
Publication status | Published - Jan 2020 |
Keywords
- 25 Hydroxyvitamin D
- Assay
- Immunoassay
- LC–MS/MS
- Mass spectrometry
- Review
- Vitamin D metabolites
Profiles
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William Fraser
- Norwich Medical School - Emeritus Professor
- Metabolic Health - Member
- Musculoskeletal Medicine - Member
Person: Honorary, Research Group Member, Research Centre Member
-
Inez Schoenmakers
- Norwich Medical School - Associate Professor
- Musculoskeletal Medicine - Member
- Nutrition and Preventive Medicine - Member
Person: Research Group Member, Academic, Teaching & Research