What’s in a name? Species-wide whole-genome sequencing resolves invasive and noninvasive lineages of Salmonella enterica Serotype Paratyphi B

Thomas R. Connor, Sian V. Owen, Gemma Langridge, Steve Connell, Satheesh Nair, Sandra Reuter, Timothy J. Dallman, Jukka Corander, Kristine C. Tabing, Simon Le Hello, Maria Fookes, Benoît Doublet, Zhemin Zhou, Theresa Feltwell, Matthew J. Ellington, Silvia Herrera, Matthew Gilmour, Axel Cloeckaert, Mark Achtman, Julian ParkhillJohn Wain, Elizabeth De Pinna, François-Xavier Weill, Tansy Peters, Nick Thomson

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Abstract

For 100 years, it has been obvious that Salmonella enterica strains sharing the serotype with the formula 1,4,[5],12:b:1,2—now known as Paratyphi B—can cause diseases ranging from serious systemic infections to self-limiting gastroenteritis. Despite considerable predicted diversity between strains carrying the common Paratyphi B serotype, there remain few methods that subdivide the group into groups that are congruent with their disease phenotypes. Paratyphi B therefore represents one of the canonical examples in Salmonella where serotyping combined with classical microbiological tests fails to provide clinically informative information. Here, we use genomics to provide the first high-resolution view of this serotype, placing it into a wider genomic context of the Salmonella enterica species. These analyses reveal why it has been impossible to subdivide this serotype based upon phenotypic and limited molecular approaches. By examining the genomic data in detail, we are able to identify common features that correlate with strains of clinical importance. The results presented here provide new diagnostic targets, as well as posing important new questions about the basis for the invasive disease phenotype observed in a subset of strains.
Original languageEnglish
Article numbere00527-16
JournalmBIO
Volume7
Issue number4
DOIs
Publication statusPublished - 23 Aug 2016

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