TY - JOUR
T1 - Wheat Pm4 resistance to powdery mildew is controlled by alternative splice variants encoding chimeric proteins
AU - Sánchez-Martín, Javier
AU - Widrig, Victoria
AU - Herren, Gerhard
AU - Wicker, Thomas
AU - Zbinden, Helen
AU - Gronnier, Julien
AU - Spörri, Laurin
AU - Praz, Coraline R
AU - Heuberger, Matthias
AU - Kolodziej, Markus C
AU - Isaksson, Jonatan
AU - Steuernagel, Burkhard
AU - Karafiátová, Miroslava
AU - Doležel, Jaroslav
AU - Zipfel, Cyril
AU - Keller, Beat
PY - 2021/3
Y1 - 2021/3
N2 - Crop breeding for resistance to pathogens largely relies on genes encoding receptors that confer race-specific immunity. Here, we report the identification of the wheat Pm4 race-specific resistance gene to powdery mildew. Pm4 encodes a putative chimeric protein of a serine/threonine kinase and multiple C2 domains and transmembrane regions, a unique domain architecture among known resistance proteins. Pm4 undergoes constitutive alternative splicing, generating two isoforms with different protein domain topologies that are both essential for resistance function. Both isoforms interact and localize to the endoplasmatic reticulum when co-expressed. Pm4 reveals additional diversity of immune receptor architecture to be explored for breeding and suggests an endoplasmatic reticulum-based molecular mechanism of Pm4-mediated race-specific resistance.
AB - Crop breeding for resistance to pathogens largely relies on genes encoding receptors that confer race-specific immunity. Here, we report the identification of the wheat Pm4 race-specific resistance gene to powdery mildew. Pm4 encodes a putative chimeric protein of a serine/threonine kinase and multiple C2 domains and transmembrane regions, a unique domain architecture among known resistance proteins. Pm4 undergoes constitutive alternative splicing, generating two isoforms with different protein domain topologies that are both essential for resistance function. Both isoforms interact and localize to the endoplasmatic reticulum when co-expressed. Pm4 reveals additional diversity of immune receptor architecture to be explored for breeding and suggests an endoplasmatic reticulum-based molecular mechanism of Pm4-mediated race-specific resistance.
UR - http://www.scopus.com/inward/record.url?scp=85102528569&partnerID=8YFLogxK
U2 - 10.1038/s41477-021-00869-2
DO - 10.1038/s41477-021-00869-2
M3 - Article
C2 - 33707738
SN - 2055-026X
VL - 7
SP - 327
EP - 341
JO - Nature Plants
JF - Nature Plants
IS - 3
ER -