Whole-cell circular dichroism difference spectroscopy reveals an in vivo-specific deca-heme conformation in bacterial surface cytochromes

Yoshihide Tokunou; Punthira Chinotaikul; Shingo Hattori; Thomas A. Clarke; Liang Shi; Kazuhito Hashimoto; Kazuyuki Ishii; Akihiro Okamoto

doi:10.1039/C8CC06309E

Whole-cell circular dichroism difference spectroscopy reveals an in vivo-specific deca-heme conformation in bacterial surface cytochromes

Yoshihide Tokunou, Punthira Chinotaikul, Shingo Hattori, Thomas A. Clarke, Liang Shi, Kazuhito Hashimoto, Kazuyuki Ishii, Akihiro Okamoto

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

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Abstract

We established whole-cell circular dichroism difference spectroscopy to identify the inter-heme interaction in deca-heme cytochrome protein MtrC in whole cell. Our data showed that the heme alignment of reduced MtrC in whole cell is distinct from that in purified one, suggesting the in vivo specific electron transport kinetics.

Original language	English
Pages (from-to)	13933-13936
Journal	Chemical Communications
Volume	54
DOIs	https://doi.org/10.1039/C8CC06309E
Publication status	Published - 7 Nov 2018

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10.1039/C8CC06309ELicence: CC BY

Published manuscriptFinal published version, 1.77 MBLicence: CC BY

http://xlink.rsc.org/?DOI=C8CC06309ELicence: CC BY

Tom Clarke
- Tom.Clarkeuea.acuk
- School of Biological Sciences - Professor
- Centre for Molecular and Structural Biochemistry - Member
- Energy Materials Laboratory - Member
- Molecular Microbiology - Member
Person: Research Group Member, Research Centre Member, Academic, Teaching & Research

Cite this

@article{4737d5e4f0834057b188a506d634751c,

title = "Whole-cell circular dichroism difference spectroscopy reveals an in vivo-specific deca-heme conformation in bacterial surface cytochromes",

abstract = "We established whole-cell circular dichroism difference spectroscopy to identify the inter-heme interaction in deca-heme cytochrome protein MtrC in whole cell. Our data showed that the heme alignment of reduced MtrC in whole cell is distinct from that in purified one, suggesting the in vivo specific electron transport kinetics.",

author = "Yoshihide Tokunou and Punthira Chinotaikul and Shingo Hattori and Clarke, {Thomas A.} and Liang Shi and Kazuhito Hashimoto and Kazuyuki Ishii and Akihiro Okamoto",

year = "2018",

month = nov,

day = "7",

doi = "10.1039/C8CC06309E",

language = "English",

volume = "54",

pages = "13933--13936",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

}

Whole-cell circular dichroism difference spectroscopy reveals an in vivo-specific deca-heme conformation in bacterial surface cytochromes. / Tokunou, Yoshihide; Chinotaikul, Punthira; Hattori, Shingo; Clarke, Thomas A.; Shi, Liang; Hashimoto, Kazuhito; Ishii, Kazuyuki; Okamoto, Akihiro.

In: Chemical Communications, Vol. 54, 07.11.2018, p. 13933-13936.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Whole-cell circular dichroism difference spectroscopy reveals an in vivo-specific deca-heme conformation in bacterial surface cytochromes

AU - Tokunou, Yoshihide

AU - Chinotaikul, Punthira

AU - Hattori, Shingo

AU - Clarke, Thomas A.

AU - Shi, Liang

AU - Hashimoto, Kazuhito

AU - Ishii, Kazuyuki

AU - Okamoto, Akihiro

PY - 2018/11/7

Y1 - 2018/11/7

N2 - We established whole-cell circular dichroism difference spectroscopy to identify the inter-heme interaction in deca-heme cytochrome protein MtrC in whole cell. Our data showed that the heme alignment of reduced MtrC in whole cell is distinct from that in purified one, suggesting the in vivo specific electron transport kinetics.

AB - We established whole-cell circular dichroism difference spectroscopy to identify the inter-heme interaction in deca-heme cytochrome protein MtrC in whole cell. Our data showed that the heme alignment of reduced MtrC in whole cell is distinct from that in purified one, suggesting the in vivo specific electron transport kinetics.

U2 - 10.1039/C8CC06309E

DO - 10.1039/C8CC06309E

M3 - Article

VL - 54

SP - 13933

EP - 13936

JO - Chemical Communications

JF - Chemical Communications

SN - 1359-7345

ER -

Whole-cell circular dichroism difference spectroscopy reveals an in vivo-specific deca-heme conformation in bacterial surface cytochromes

Abstract

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Tom Clarke

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