TY - JOUR
T1 - Zinc octa-n-alkyl phthalocyanines in photodynamic therapy: photophysical properties, accumulation and apoptosis in cell cultures, studies in erythrocytes and topical application to Balb/c mice skin
AU - Kaestner, Lars
AU - Cesson, Maro
AU - Kassab, Kawser
AU - Christensen, Terje
AU - Edminson, Paul D.
AU - Cook, Michael J.
AU - Chambrier, Isabelle
AU - Jori, Giulio
PY - 2003
Y1 - 2003
N2 - Two octa-substituted phthalocyanines, namely 1,4,8,11,15,18,22,25-octakis(decyl) phthalocyaninato zinc(II) (ZnODPc) and 1,4,8,11,15,18,22,25-octakis(pentyl) phthalocyaninato zinc(II) (ZnOPPc), were investigated for their use in photodynamic therapy ( PDT) after topical application. Both substances exhibited favourable properties as photosensitisers in vitro: absorption maxima around 700 nm with absorption coefficients of about 190000 (M-1 cm(-1)), a singlet oxygen quantum yield of 0.47 +/- 0.02 (ZnODPc), and good accumulation in keratinocytes and. broblasts. Cell death after phthalocyanine-photosensitisation appeared to occur mainly via apoptosis. The in vivo experiments demonstrated a good accumulation of the phthalocyanines after topical application in a tetrahydrofuran - azone formulation onto the dorsal skin of Balb/c mice: [(4.6 - 4.7) +/- 1.0]% of deposited dye could be recovered after 3 h from deposition. ZnODPc showed significantly better skin-photosensitising properties than ZnOPPc and is therefore a potential candidate for the treatment of psoriasis.
AB - Two octa-substituted phthalocyanines, namely 1,4,8,11,15,18,22,25-octakis(decyl) phthalocyaninato zinc(II) (ZnODPc) and 1,4,8,11,15,18,22,25-octakis(pentyl) phthalocyaninato zinc(II) (ZnOPPc), were investigated for their use in photodynamic therapy ( PDT) after topical application. Both substances exhibited favourable properties as photosensitisers in vitro: absorption maxima around 700 nm with absorption coefficients of about 190000 (M-1 cm(-1)), a singlet oxygen quantum yield of 0.47 +/- 0.02 (ZnODPc), and good accumulation in keratinocytes and. broblasts. Cell death after phthalocyanine-photosensitisation appeared to occur mainly via apoptosis. The in vivo experiments demonstrated a good accumulation of the phthalocyanines after topical application in a tetrahydrofuran - azone formulation onto the dorsal skin of Balb/c mice: [(4.6 - 4.7) +/- 1.0]% of deposited dye could be recovered after 3 h from deposition. ZnODPc showed significantly better skin-photosensitising properties than ZnOPPc and is therefore a potential candidate for the treatment of psoriasis.
U2 - 10.1039/b211348a
DO - 10.1039/b211348a
M3 - Article
SN - 1474-905X
VL - 2
SP - 660
EP - 667
JO - Photochemical & Photobiological Sciences
JF - Photochemical & Photobiological Sciences
IS - 6
ER -